Background & Aims

The TNBS model is widely used to mimic colitis in rodents and represents a form of inflammatory bowel disease (IBD). This study employs a dual approach, combining in vivo behavioral assessments and ex vivo molecular analyses, focusing on non-evoked pain and molecular markers using qPCR on colon samples.

Methods

Female Sprague-Dawley rats underwent TNBS-induced colitis, with non-evoked pain monitored on days 4 and 7. Colon tissues, harvested 7 days post-TNBS exposure, underwent macroscopic damage scoring and qPCR analysis. TNBS induced noticeable pain, quantified through eye closure and postures, and modestly increased the DAI on days 4 and 7.

Results

The administration of TNBS also resulted in an elevated damage score observed through macroscopic evaluation of the colon and heightened cytokine levels in the colon, as measured on day 7.

Conclusions

Integrating in vivo assessments together with ex vivo readouts, enhances understanding and facilitates the evaluation of potential analgesic/anti-inflammatory treatments for TNBS-induced colitis, holding promise for the development of targeted therapeutic interventions addressing the complex pathophysiology of IBD.

References

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Witaicenis A, Luchini AC, Hiruma-Lima CA, Felisbino SL, Garrido-Mesa N, Utrilla P, Gálvez J, Di Stasi LC. Suppression of TNBS-induced colitis in rats by 4-methylesculetin, a natural coumarin: comparison with prednisolone and sulphasalazine. Chem Biol Interact. 2012 Jan 5;195(1):76-85. doi: 10.1016/j.cbi.2011.11.004.

Zhao HM, Wang Y, Huang XY, Huang MF, Xu R, Yue HY, Zhou BG, Huang HY, Sun QM, Liu DY. Astragalus polysaccharide attenuates rat experimental colitis by inducing regulatory T cells in intestinal Peyer’s patches. World J Gastroenterol. 2016 Mar 21;22(11):3175-85. doi: 10.3748/wjg.v22.i11.3175

Presenting Author

Sabrina Djemmali

Poster Authors

Solène Pedron

Physiology/Neurosciences

Université de Franche Comté

Lead Author

Topics

  • Specific Pain Conditions/Pain in Specific Populations: Visceral Pain – Gastrointestinal/Abdominal