Background & Aims
The TNBS model is widely used to mimic colitis in rodents and represents a form of inflammatory bowel disease (IBD). This study employs a dual approach, combining in vivo behavioral assessments and ex vivo molecular analyses, focusing on non-evoked pain and molecular markers using qPCR on colon samples.
Methods
Female Sprague-Dawley rats underwent TNBS-induced colitis, with non-evoked pain monitored on days 4 and 7. Colon tissues, harvested 7 days post-TNBS exposure, underwent macroscopic damage scoring and qPCR analysis. TNBS induced noticeable pain, quantified through eye closure and postures, and modestly increased the DAI on days 4 and 7.
Results
The administration of TNBS also resulted in an elevated damage score observed through macroscopic evaluation of the colon and heightened cytokine levels in the colon, as measured on day 7.
Conclusions
Integrating in vivo assessments together with ex vivo readouts, enhances understanding and facilitates the evaluation of potential analgesic/anti-inflammatory treatments for TNBS-induced colitis, holding promise for the development of targeted therapeutic interventions addressing the complex pathophysiology of IBD.
References
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Witaicenis A, Luchini AC, Hiruma-Lima CA, Felisbino SL, Garrido-Mesa N, Utrilla P, Gálvez J, Di Stasi LC. Suppression of TNBS-induced colitis in rats by 4-methylesculetin, a natural coumarin: comparison with prednisolone and sulphasalazine. Chem Biol Interact. 2012 Jan 5;195(1):76-85. doi: 10.1016/j.cbi.2011.11.004.
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Presenting Author
Sabrina Djemmali
Poster Authors
Solène Pedron
Physiology/Neurosciences
Université de Franche Comté
Lead Author
Topics
- Specific Pain Conditions/Pain in Specific Populations: Visceral Pain – Gastrointestinal/Abdominal