Background & Aims
Oxygen-ozone (O2-O3; 95% O2 and 5% O3) therapy has gained interest in treating pain. However, its effect and mechanisms have not been investigated in temporomandibular (TMJ) osteoarthritis pain. This study hypothesized that the TMJ injection of the O2-O3 therapy reduces TMJ osteoarthritis pain by reducing TMJ inflammatory IL-1?, TNF-? cytokines and releasing endogenous opioids.
Methods
Experiments were conducted in Wistar female rats (220-280g) and approved by the Ethics Committee in Animal Research at the UNICAMP. Monosodium Iodoacetate (MIA; 2mg/ 30 ?l)-induced TMJ osteoarthritis pain was evaluated by measuring the mechanical nociceptive thresholds with an electronic von Frey. Three days after TMJ-MIA injection, when the TMJ mechanical nociceptive thresholds significantly decreased, the O2-O3 (40 ?g/ml, 50 ?l) was injected into the TMJ. On day nine post-MIA injection, when mechanical nociceptive thresholds had returned to baseline levels, the non-selective opioid receptor antagonist naloxone (0.1 mg/30?l) or saline was injected into the TMJ and the mechanical nociceptive threshold was measured 15 minutes later. ELISA measured IL-1? and TNF-? cytokines in joint tissues on day seven post-MIA injection. One- or two-way ANOVA followed by the Tukey or Dunnett test was performed as appropriate (p<0.05; 6-8 rats/group).
Results
The TMJ injection of MIA induced mechanical hyperalgesia, as shown by the significant decrease (44%) in the mechanical nociceptive thresholds three days later. The TMJ injection of O2-O3 gas mixture (40 ?g/ml, 50 ?l) significantly reduced MIA-induced TMJ hyperalgesia as evidenced by the increase in the mechanical nociceptive threshold (from Mean±EPM:56.6 ±14.2 to 88.5 ±8.8) at day seven post-MIA injection. The TMJ injection of Naloxone significantly reduced this analgesic effect of O2-O3 (84.9 ±4.2) since the mechanical nociceptive thresholds significantly decreased (66.9 ± 7.1) 15 minutes post Naloxone injection. Naloxone by itself did not affect the mechanical threshold in the TMJ saline control group. The TMJ injection of the O2-O3 mixture significantly reduced the release of the inflammatory cytokines IL-1? (41%) and TNF-? (65%).
Conclusions
O2-O3 therapy reduces TMJ osteoarthritis pain due to an anti-inflammatory action and the release of endogenous opioids.
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Presenting Author
Tássia Tillemont Machado
Poster Authors
Tássia Machado
OTHR
UNICAMP
Lead Author
Ana Carolina Machado
Universidade Estadual De Campinas (UNICAMP)
Lead Author
Carlos A. Parada
State University of Campinas (UNICAMP)
Lead Author
César Sartori
BPE
Universidade Estadual de Campinas - UNICAMP
Lead Author
Cláudia Tambeli
State University of Campinas (UNICAMP)
Lead Author
Topics
- Mechanisms: Biological-Molecular and Cell Biology